- Know general features about enzymes: how do they cause such great catalysis (transition state) and bind with specificity.

- Know the Michaelis-Menten equation. By inspection of the data, determine K_M, V_max, k_cat, and estimation of catalytic efficiency (specificity constant) ( k_cat/K_M). Know the meaning of the above.

- Know how the “catalytic triad” of chymotrypsin works. Know how the experimental data (irreversible inhibition, affinity labeling and biphasic kinetics) work and support the mechanism of chymotrypsin.