Example of a sequencing problem “2”:

I.          Octapeptide aa composition (in alphabetical order): Ala₂, Arg, Gly, Lys, Phe, Trp, Val

II.         Treatment with fluorodinitrobenzene derivatizes Gly.

            ___  ___  ___  ___  ___  ___  ___  ___ 

III.       Treatment with trypsin yields 2 products with the following aa composition:

            1) Lys, Val, Trp

            2) Ala₂, Arg, Gly, Phe

___  ___  ___  ___  ___  ___  ___  ___

IV.       Treatment with pepsin yields 3 products with the following aa composition:

            3) Gly

            4) Lys, Val, Trp

            5) Ala₂, Arg, Phe

            ___  ___  ___  ___  ___  ___  ___  ___

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